QTL Mapping of Somatic Regeneration-Related Traits in Maize
نویسندگان
چکیده
The somatic regeneration of maize depends on its genotypes, so improving variety with modern biotechnology is severely restricted. Locating the quantitative trait loci (QTLs) associated important for breeding elite inbred lines that undergo genetic transformations. Here, by crossing high-regeneration line H99 and non-regeneration Fr993, an F2 population F2:3 F2:4 families were constructed. Immature embryos from family populations subjected to tissue culture in two independent seasons determine their embryogenic callus induction rates (EIRs), green (GCRs) plantlet (PRRs). Genetic linkage maps constructed locate QTLs. results showed variation EIR, GCR PRR ranged 0.00–99.33%, broad-sense heritabilities 0.50, 0.52 0.53, respectively. total distance GenoBaits 10 K chip was 2319.50 cM, twelve QTLs traits, accounting 3.90–14.06% phenotypic variation. Expression analysis revealed six candidate genes screened distinct responses parental lines, suggesting they may impact commitment cell fate. These provide a basis molecular varieties high-frequency regeneration.
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ژورنال
عنوان ژورنال: Agriculture
سال: 2022
ISSN: ['2077-0472']
DOI: https://doi.org/10.3390/agriculture12030393